AmScope IN400T-5M Digital Long Working-Distance Inverted Trinocular Microscope, 40x-600x, WH10x Super-Widefield Eyepieces, Brightfield and Phase-Contrast Objectives, 30W Halogen Illumination, 0.3 NA Abbe Condenser, Plain Stage, 90 to 240V, Includes 5MP Camera with Reduction Lens and Software

The AmScope IN400T-5M digital inverted trinocular compound microscope has a pair of 10x22mm high-eyepoint super-widefield eyepieces, an under-mounted quadruple nosepiece, eight DIN long working-distance infinity plan objectives, top-mounted Brightfield 30W halogen illumination, a 0.3 NA Abbe condenser with iris diaphragm, and a plain stage with a stage stop to give protection to slides and objectives from damage. The 5MP camera has a CMOS color sensor, a reduction lens, image capture and editing software, and USB 2.0 output to capture or display still or video images on a pc or projector. The microscope has a color-corrected infinity optical system (CCIS) that provides color correction and improved focal point of the intermediate image across all the focal plane. The trinocular head has a Siedentopf binocular mount with 50 to 75mm interpupillary adjustment and a fixed 45-degree vertical inclination to reduce eye and neck strain. A Siedentopf binocular head enables the viewer to change the interpupillary distance without changing the tube length, getting rid of the wish to refocus the image. Anti-mold coatings give protection to the microscope in high-humidity environments. A pair of WH10x high-eyepoint super-widefield eyepieces provides improved focal point over all the field of view (FOV). High-eyepoint eyepieces ease viewing for viewers who wear glasses. Dioptric adjustment on both eyepieces incorporates individual eye-strength differences. The vertical trinocular port accepts a 23mm or C-Mount camera. The under-mounted quadruple nosepiece accepts as much as four 20mm objectives at a time. The microscope includes five Brightfield long working-distance infinity plan objectives (4x, 10x, 20x, 40x, and 60x) and three phase-contrast long working-distance infinity plan objectives (10x, 20x, and 40x). Long working-distance plan objectives provide improved focal point over all the range of the viewing field and a longer working distance that may be required when viewing specimens in dishes and large containers. Phase-contrast provides high contrast and visibility without using stains, allowing specimens to be observed of their natural state without being killed or fixed. An inverted microscope has its light source above the stage pointing downward and the objectives and turret below the stage pointing upward. It’s most regularly used for observing organisms in a big container, such as a well plate or petri dish. It may also be used when specimens require manipulation, or in metallurgical applications.

The 5MP digital camera has a CMOS color sensor for displaying still microscopy images and streaming live videos to a pc or projector. The camera has 40x magnification and a 0.5x reduction lens that ensures that the display has a equivalent field of view as the microscope eyepiece. The camera can also be mounted in a C-Mount or any 23mm eye tube. The camera includes image capture and editing software that provides still image and live video capture and editing capability, including measurement functions. The software supports JPG, TIF, GIF, PSD, WMF, and BMP file formats and is compatible with Windows XP, Vista, 7, and 8; Mac OS X; and Linux. Camera drivers are compatible with Windows XP, Vista, 7, and 8; Mac OS X; and Linux. The software includes Windows APIs for native C/C++, C#, DirectShow, Twain, and LabVIEW that enable custom application development. The camera has a USB 2.0 data port (cable included).

Brightfield and phase-contrast illumination is mounted above the stage and has transmitted 30W halogen illumination. Brightfield (BF) illumination allows the specimen to take in light, resulting in a dark image on a light background. The phase-contrast element aligns the light source with the phase-contrast objectives to optimize high contrast and visibility without using stains. Halogen illumination provides bright light in a concentrated path, and a rheostat controls the amount of light emanating from the lamp. The movable 0.3 NA Abbe condenser can also be adjusted to regulate the distance of the light from the stage, and the iris diaphragm has an opening that focuses and controls the amount of light illuminating the specimen from fully open to fully closed. The oversize stage is 6.3 x 10 inches (160 x 250mm) (W x D, where W is width, the horizontal distance from left to right; D is depth, the horizontal distance from front to back). Nested coaxial coarse and fine focal point has tension-adjustable coarse focusing and an adjustable lock ring stopper that limits stage range to give protection to slides and objectives from damage. All-metal mechanical parts, solid-metal frame construction, and a stain-resistant enamel finish provide durability.

Microscopes are instruments used to beef up the resolution of an object or image. Types include compound, stereo, or digital. Compound microscopes use a compound optical system with an objective lens and an eyepiece. Stereo microscopes show object depth in a three-dimensional image. Digital microscopes are used to display an image on a monitor, slightly than having a look through a lens. Microscopes could have monocular (one), binocular (two), or trinocular (three) eyepieces, with varying magnification abilities. Magnification ability refers back to the size of an image. Resolution, often referred to as resolvant power, refers back to the clarity of the image. The interaction between field of view (FOV), numerical aperture (NA), and working distance (WD) determines resolution. Microscopes can regulate magnification through a fixed focal point, or through a range of adjustments. They may be able to also utilize LED, fluorescent, and mirror light sources to assist regulate viewing capabilities. Microscopes are widely used in education, lab research, biology, metallurgy, engineering, chemistry, manufacturing, and within the medical, forensic science, and veterinary industries.

United Scope manufactures microscopy equipment and accessories under the brand name AmScope. The company, founded in 1996, is headquartered in Irvine, CA.

What’s within the Box?

• AmScope IN400T-5M microscope
• WH10x22mm plan eyepieces, 30mm, one pair
• 4x DIN long working-distance infinity plan objective, 20mm
• 10x DIN long working-distance infinity plan objective, 20mm
• 20x DIN long working-distance infinity plan objective, 20mm
• 40x DIN long working-distance infinity plan objective, 20mm
• 60x DIN long working-distance infinity plan objective, 20mm
• 10xPH DIN long working-distance infinity plan phase-contrast objective, 20mm
• 20xPH DIN long working-distance infinity plan phase-contrast objective, 20mm
• 40xPH DIN long working-distance infinity plan phase-contrast objective, 20mm
• Color filters
• 5MP digital camera (MU500)
• 0.5x reduction lens
• USB 2.0 cable
• Software CD
• Reflected illuminations system with top light housing and electrical cord
• Episcopic light box and tube
• Power cord
• Dust cover
• Instructions